Cell Imaging Multi-Mode Reader
[CYT5MPV, BioTek Instruments, USA]

Located at: CRF Annexe - S01

I-STEM Equipment Code : 2938918 (This code can be used to locate the equipment on the I-STEM portal)

  • Images

Facilities:

Module supports detection modes: UV-Vis absorbance, Fluorescence Luminescence, Time-resolved fluorescence

Read methods: Endpoint, kinetic, spectral scanning, well area scanning.

Applications:

• Absorbance, fluorescence and luminescence-based endpoint and kinetic assays

• Cell imaging: 6 to 1536 well plates

• Determination of cell count

• Cytoplasm, intracellular, subpopulation analysis,

• Signal translocation

• Cell migration and invasion

• Immunofluorescence

• Phenotypic assays

• Histology

Technical specifications Cell imaging multimode reader

• Detection mode: Fluorescence, Luminescence, UV-Vis absorbance and Time-resolved fluorescence

• Light sources: Xenon flash

• Detector: PMT for florescence system, Photodiode for absorbance

• Wavelength range: 250-700nm (fluorescence), 230-999nm (absorbance)

• Dynamic range: 7 decades (fluorescence), 0.4 OD (absorbance), >6 decades (luminescence)

• Reading speed (kinetic): 96 wells in 11 seconds, 384 wells in 22 seconds

• Assay type: End point, kinetic, spectral and well scanning read

• Plate compatibility: Reads 6 to 384 well plates and imaging of 6 to 1536 well plates

• It can accommodate microscope slides, petri and cell culture dishes, cell culture flasks (T25)

• Plate shaking: Linear, orbital, double orbital shaking modes

• Imaging modes: Fluorescence, bright-field, color bright-field and phase contrast with single color, multi-color, montage, time lapse and z-stacking

• Image filter cube: DAPI, GFP, Texas Red Filter cubes with imaging LED cubes of 365nm, 465nm and 590nm

• Monochromator wavelength accuracy and repeatability:2 nm, +0.2 nm

• OD accuracy: <1% at 2.0 OD <3% at 3.0 OD, OD linearity; <1% from 0 to 3.0 OD; OD repeatability:<0.5% at 2.0 OD

Testing charges.

Plate reading assay [per sample/plate] Cell imaging [per sample per mode]
NITK Rs. 300/- Rs. 600/-
Academic Institute* Rs. 660/- Rs. 960/-
R&D Institute* Rs. 780/- Rs. 1200/-
Industry* Rs. 900/- Rs. 1440/-

*Sample preparation charges, GST(18%) extra. Above charges are for providing raw data.

For further Query Contact:

Ms. Jayashree Arun (Technical Staff, CRF), Email: crf_cellimg@nitk.edu.in

Dr.(Ms) Gangamma S (Cell-Imaging Faculty in-charge), Email: gangamma@nitk.edu.in, Mob: +91-8970110470

Prof. Keyur Raval, Professor InCharge-CRF, Email: keyurnraval@nitk.edu.in, Mob: +91-9342421444



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